Composite
Part:BBa_K1585311:Experience
Designed by: Sarah Lubjuhn, Laura Helleckes, Michael Osthege Group: iGEM15_Aachen (2015-08-25)
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Applications of BBa_K1585311
The construct was confirmed by sequencing. The expression was tested in Bl21 Gold (DE3) strains containing BBa_K1585311 in a pSB1K30 expression vector after IPTG induction.
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| SDS-PAGE of GlgB in comparison to mRFP After induction with IPTG at OD=0.6, glgB was expressed. Samples were taken 6 and 19.5 hours after induction. The small arrows indicates the expected bands for GlgB. mRFP in pSB1K30 (T7 promoter) was used as negative control. |
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